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KMID : 0379119950230030202
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Korean Journal of Mycology
1995 Volume.23 No. 3 p.202 ~ p.208
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Parameters Affecting Polymerase Chain Reaction in RAPD Analysis of Pleurotus spp.
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이창수/Lee, Chang Soo
류진창/김범기/유영복/정미정/이희경/Ryu, Jin Chang/Kim, Beom Gi/Yoo, Young Bok/Jeong, Mi Jeong/Lee, Hee Kyung
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Abstract
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This study describes the effects of several components on PCR amplification used for RAPD. We used different concentrations of reaction components to obtaine discrete and reproducible PCR products from Pleurotus cornucopiae. The optimum concentrations of reaction components were found to be 80 ng of template DNA, 30 pmole of 10-mer primer, 200 μM dNTP, 2 mM MgCl₂, 50 mM KCl, 10 mM Tris-HCl (pH 9.0), 0.1% Triton X-100, 1.5 unit of Taq DNA polymerase (promega) in 50 Nl reaction volume. The optimum annealing temperature was 35℃. These results proved to be valuable for characterization of Pleurotus spp.
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